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. 2009 Sep 14;4(9):e7030. doi: 10.1371/journal.pone.0007030

Figure 2. Gemin5 isolated by cap-affinity is not bound to the SMN complex.

Figure 2

(A) Schematic representation of gemin5 and eIF4E indicating locations of WD repeats, the putative coiled coil domain, and epitope tags. (B) 293T cells were co-transfected with expression constructs encoding FLAG-tagged gemin5 and myc-tagged eIF4E. Cell lysate was derived 24 hours later and applied to cap-sepharose 4B or sepharose 4B. Tagged proteins were detected with α-FLAG and α-myc antibodies. (C) Purification of proteins by cap-affinity was performed as in figure 1A and levels of endogenous gemin3–5 were examined in input, supernatant and precipitate samples.