Figure 7. Gemin5 mutations that affect m⁷G interaction reduce association with U1 snRNA.

(A) Gemin5-FLAG and the indicated variants were transiently expressed in 293T cells and then immunoprecipitated with α-FLAG antibody or negative control mouse IgG. A 10% fraction of IP samples along with input samples were subjected to α-FLAG western blot. The asterisk indicates a cross-reactive band that serves as a loading control. The remaining IPs were used for RNA extraction. (B) RT-qPCR was performed on extracted RNAs for measurements of U1 and U6 snRNA levels in positive and negative IP samples for each gemin5 variant. Error bars indicate values for standard deviation.