Figure 7.

A comparison between SHG imaged structures of unstretched (a) and stretched (b) muscle cells from Drosophila melanogaster larva. The arrows point to the same sarcomere which appears in its double-banded form in the nonstretched myocyte (a), and in its single banded structure for the stretched myocyte (b). A line profile of the indicated (arrow) sarcomeres is plotted in (c) showing that unstretched sarcomeres have a double peak and stretched sarcomeres have a single peaked A-band. The anisotropic muscle regions, indicated by A, produce SHG; while the isotropic muscle regions, indicated by I, show no SHG. The position of the M-line is indicated by M. The scale bar is 8 μm.