Table 1. Fraction of Apelin-17 Bound (fb) to the Indicated Micelle Types as Determined through Diffusion-Ordered NMR Spectroscopy Methodsa.
| component | Δ (ms) | δ (ms) | Dobsd (m2/s) | fb |
|---|---|---|---|---|
| SDS micelles | 60 | 6.4 | 1.7 × 10−10 ± 1.6 × 10−12 | N/A |
| DPC micelles | 60 | 6.4 | 1.8 × 10−10 ± 1.6 × 10−12 | N/A |
| LPPG micelles | 85 | 7.0 | 9.6 × 10−11 ± 2.8 × 10−13 | N/A |
| apelin-17 | 55 | 4.4 | 3.6 × 10−10 ± 3.4 × 10−12 | N/A |
| apelin-17 with SDS micelles | 65 | 5.2 | 2.0 × 10−10 ± 1.2 × 10−11 | 82 ± 5% |
| apelin-17 with DPC micelles | 60 | 5.2 | 2.8 × 10−10 ± 3.4 × 10−12 | 46 ± 5% |
| apelin-17 with LPPG micelles | 70 | 6.6 | 1.0 × 10−10 ± 1.2 × 10−12 | 98 ± 5% |
a
Topspin (Bruker) was used to determine the diffusion coefficients (Dobsd) of three peaks of the component of interest for three replicate experiments to give an average Dobsd with associated average deviation. Assuming two-state fast exchange for apelin-17, fb was estimated using eqs 3 and 4. The experimental settings for the diffusion time (Δ) and gradient duration (δ) for each experiment are indicated; pulsed field gradients were varied from 1.7 to 32.0 G/cm.