Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 18;5:295. doi: 10.1038/msb.2009.55

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, EMBO and Nature Publishing Group

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits distribution and reproduction in any medium, provided the original author and source are credited. This licence does not permit commercial exploitation or the creation of derivative works without specific permission.

PMC Copyright notice

UBE2U physically interacts with MDM2. (A) HEK293T cells were transiently transfected with myc-tagged MDM2, lysed and incubated with GST–E2s immobilized on gluthathion-agarose beads. Bound material was resolved on 7.5% SDS–PAGE and proteins were visualized after immunoblotting with antibodies against myc. (B) Untransfected U2OS cells were lysed and combined with GST–E2s as in panel A. Immunoblotting was carried out using MDM2 antibodies (upper panel) and using p53 antibodies after reprobing (lower panel). Arrow indicates MDM2 signal, asterisk indicates background band.