Figure 3.

Activation of EP receptors in podocyte injury. (A) Puromycin stimulated EP₄ and TP mRNA expression in podocytes from podCOX-2-tg mice. RNA was isolated from cultured differentiated podocytes in each group with or without PAN administration: EP₁, EP₄, TP, and FP (PGF_2α receptor). mRNA expression were measured by real-time RT-PCR as described in Concise Methods (n = 4; *P < 0.05 compared with basal level or wild type + PAN). (B) Puromycin-induced EP₄ and TP expression in cultured podocytes from podCOX-2-tg mice. EP₁, EP₄, FP, and TP from differentiated podocytes were measured with their specific antibodies by immunoblotting (detail in Concise Methods). The photographs are representative of three separate experiments. (C) Adriamycin upregulated glomerular EP₄ and TP mRNA in podCOX-2-tg mice. Glomeruli were isolated from wild-type and podCOX-2-tg mice with or without adriamycin injection as described in Concise Methods. Expression of EP₁, EP₄, TP, and FP mRNA were tested as in (A) (n = 4; *P < 0.05 compared with basal level or wild type + adriamycin). (D) Adriamycin stimulated glomerular EP₄ and TP expression in podCOX-2-tg mice. EP₁, EP₄, FP, and TP from isolated glomeruli were measured with their specific antibodies by immunoblotting as in (B). The photographs are representative of three separate experiments.