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Endothelioma cell migration is not influenced by HPSE-degraded HS but VEGF stimulates wound healing. To study how exogenous addition of HS will influence endothelioma (b.End3) biological activity, we added HPSE-digested melanoma cell surface HS to serum-free endothelioma medium. HS treatment did not stimulate endothelioma cell migration while VEGF did (p< 0.05), as expected. HS, when added along with VEGF, did not augment VEGF response.
