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HPSE-degraded cell surface HSGAG does not influence melanoma cell proliferation. Proliferation of melanoma cells were assayed by alamarBlue™, a non-toxic dye that monitors the reducing environment of the proliferating cell. Melanoma cell proliferation was monitored every 24 h for 72 h. Exogenous addition of VEGF (50 ng/ml) or melanoma cell surface HS (1 ng/ml) isolated by HPSE treatment did not influence cell proliferation.
