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. 2009 Aug 25;101(5):829–839. doi: 10.1038/sj.bjc.6605208

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Copyright © 2009 Cancer Research UK

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YA1 stimulates GJIC between transformed and non-transformed cells. (A) Cells derived from a single GEF16 transformed colony were electroporated with LY and co-cultured with non-transformed cells that had been labelled earlier with PKH67. Either YA1 (10 μM) or DMSO control was added to duplicate cultures and these were collected at T=0, 1.5, 4.5 and 7.5 h for analysis by flow cytometry. The numbers of cells displaying each type of fluorescence is shown. (B) The ratio of double LY/PKH67-labelled cells expressed as percentage of the total LY population.