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. 2009 Aug 25;122(18):3393–3402. doi: 10.1242/jcs.045427

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Fig. 4. — Colocalisation studies of NIS, CD63, PBF and caveolin. (A) Cellular expression of transiently transfected NIS-HA (i) and PBF-HA (ii) (both red) with endogenous CD63 (green), a tetraspanin and a marker of late endosomes, in COS-7 cells. Endogenous CD63 staining in late endosomes is coincident with that of both NIS and PBF, as indicated by yellow staining in merged images. (iii) Relationship between PBF-HA (green), NIS-HA (green) and caveolin-1 (red), a marker of caveolae-dependent lipid trafficking and endocytosis. Neither PBF nor NIS colocalises with caveolin in COS-7 cells. (B) (i) Confocal microscopy was used to confirm the colocalisation of NIS and PBF with CD63, shown in yellow. (ii) Confocal images of NIS, PBF and caveolin in COS-7 cells. Nuclei are visualised in blue with Hoechst 33342 stain in the merged images. Scale bars: 20 μM.