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. 2009 Aug 25;122(18):3393–3402. doi: 10.1242/jcs.045427

Fig. 6.

Fig. 6.

The effect of PBF on NIS function. (A) Examination of the subcellular localisation of WT and mutated HA-tagged PBF in COS-7 cells (i), Myc-tagged NIS (ii) and colocalisation (iii). WT PBF predominantly colocalises with NIS within late endosomes. M1 colocalises with NIS at the cell membrane, whereas M2 and M3 fail to show any significant colocalisation with NIS. (B) Confocal images demonstrating the localisation of WT and mutant PBF in FRTL-5 cells. Scale bars: 20 μM. (C) Iodide-uptake studies in rat thyroid FRTL-5 cells transfected with WT PBF and mutants M1, M2 and M3. Graph shows c.p.m. values normalised to that of the VO control. ***P<0.001.