Table 1.
Summary of genes with significant (P <0.05) differential expression (>2.0-fold) between heat-stressed and control maturing microspores
| Affy ID | Accession/AGI no.* | E-value† | Gene description‡ | Arabidopsis orthologue§ | Fold¶ |
| Stress response | |||||
| HSPs | |||||
| LesAffx.69957.1.S1_atBH | AT1G52560 | 8E-50 | 26.5 kDa class I small heat shock protein-like (AtHSP26.5-P-CI); A. thaliana | 158 | |
| Les.269.1.S1_atBH | U66300 | 1E-132 | Chloroplast heat shock protein (LeHSP21); S. lycopersicum | AT4G27670 | 155 |
| Les.3739.1.S1_atBH | AB026983 | 5E-116 | Endoplasmic reticulum-located small heat shock protein (LeERHSP21.5); S. lycopersicum | AT4G10250 | 154 |
| LesAffx.10596.1.S1_atBH | AT5G59720 | 1E-63 | 18.2kDa heat shock protein (AtHSP18.2); A. thaliana | 141 | |
| Les.3677.1.S1_atBH | AY128100 | 1E-134 | Small heat shock protein (vis1); S. lycopersicum | AT4G27670 | 132 |
| Les.4004.1.S1_a_atBH | AF123256 | 2E-66 | 17.8 kDa class I small heat shock protein (LeHSP17.8-CI); S. lycopersicum | AT1G07400 | 93 |
| Les.4150.1.S1_atBH | AB017134 | 2E-131 | Mitochondrial small heat shock protein (LeMTHSP23.8); S. lycopersicum | AT4G25200 | 82 |
| Les.3578.1.S1_atBH | AF090115 | 2E-99 | Cytosolic class II small heat shock protein HCT2 (LeHSP17.4-CII); S. lycopersicum | AT5G12020 | 75 |
| LesAffx.70264.1.S1_atBH | AB333795 | 6E-49 | Peroxisomal small heat shock protein (GmPHS); Glycine max | AT5G37670 | 64 |
| Les.3550.1.S1_atBH | AF096251 | 7E-7 | Ethylene-responsive heat shock protein cognate 70 (LeHSC70/ER21); S. lycopersicum | 83 | |
| LesAffx.56637.1.S1_atBH | AT3G09700 | 2E-29 | DNAJ heat shock N-terminal domain-containing protein; A. thaliana | 70 | |
| LesAffx.34336.1.S1_atBH | AT2G32120 | 1E-112 | HSP70T-2; A. thaliana | 36 | |
| LesAffx.10807.1.S1_at | X13301 | 3E-29 | Heat shock protein hsp70 (PhHSP70); Petunia×hybrida | AT5G02490 | 7 |
| Les.3134.1.S1_at | AF123259 | 7E-60 | Heat shock protein 90 (LeHSP90); S. lycopersicum | 5 | |
| Oxidative stress | |||||
| Les.5622.1.S1_atBH | AT3G16050 | 1E-143 | Pyridoxal phosphate synthase protein (AtPDX1); A. thaliana | 64 | |
| LesAffx.3918.1.S1_atBH | DQ096286 | 4E-108 | Cytosolic ascorbate peroxidase 3 (SlAPX3); S. lycopersicum | AT3G09640 | 53 |
| Les.1132.1.A1_atBH | AT1G17870 | 2E-43 | Ethylene-dependent gravitropism-deficient and yellow-green-like (ATEGY3); A. thaliana | 32 | |
| Les.4709.1.S1_at | AT2G27680 | 0 | Aldo/keto reductase family protein; A. thaliana | 6 | |
| Les.4223.1.S1_at | AY034148 | 0 | Alternative oxidase 1a (LeAOX1a); S. lycopersicum | AT3G22370p | 5 |
| Les.1724.1.S1_at | AB087837 | 9E-65 | Glutathione S-transferase (PsGST); Pisum sativum | AT5G02790 | 4 |
| Les.1724.2.S1_at | AI776156 | 1E-12 | Glutathione S-transferase (PsGST); Pisum sativum | AT5G02790 | 4 |
| Others | |||||
| LesAffx.47187.1.S1_atBH | AT3G03270 | 4E-55 | Universal stress protein (USP) family protein/early nodulin ENOD18 family protein; A. thaliana | 28 | |
| Hormone metabolism and response | |||||
| Ethylene | |||||
| Les.3642.1.S1_at | U17972 | 0 | 1-Aminocyclopropane-1-carboxylate synthase 3/ACC synthase 3 (LeACS3); S. lycopersicum | AT4G37770 | 6 |
| Les.3766.1.S1_at | U77719 | 1E-108 | Ethylene-responsive late embryogenesis-like protein (ER5); S. lycopersicum | AT2G46140p | 4 |
| Abscisic acid | |||||
| Les.4807.1.S1_atBH | AT5G13200 | 1E-111 | ABA-responsive protein-related/GRAM domain-containing protein; A. thaliana | 25 | |
| Jasmonic acid | |||||
| Les.368.1.S1_atBH | DQ359730 | 6E-34 | Jasmonate resistance 1-like protein (NaJAR6); Nicotiana attenuate | AT2G46370 | 43 |
| Defence | |||||
| LesAffx.66384.1.S1_atBH | AT1G12060 | 2E-20 | BCL-2-associated athanogene 5 (AtBAG5); A. thaliana | 56 | |
| LesAffx.58019.1.S1_atBH | AT5G20740 | 1E-29 | Invertase/pectin methylesterase inhibitor family protein; A. thaliana | 44 | |
| LesAffx.1881.1.S1_atBH | AT4G36010 | 6E-73 | Pathogenesis-related thaumatin family protein; A. thaliana | 19 | |
| Carbohydrate metabolism | |||||
| Les.3522.1.S1_at | AF071786 | Sucrose-phosphate synthase; S. lycopersicum | AT5G20280 | 7 | |
| Les.3069.1.S1_at | AT2G22900 | 4E-22 | Galactosyl transferase GMA12/MNN10 family protein; A. thaliana | 5 | |
| Les.3696.1.S1_at | AF311943 | 0 | Galactinol synthase 1 (LeGolS-1); S. lycopersicum | AT2G47180 | 4 |
| LesAffx.10299.1.A1_at | DQ104196 | 2E-77 | Sorbitol transporter (ST1); Nicotiana langsdorffii×Nicotiana sanderae | AT3G18830 | 4 |
| Transcription regulation | |||||
| LesAffx.24696.1.S1_atBH | X67601 | 2E-119 | Heat stress transcription factor 30 (LpHSF30/HSFA2); L. peruvianum | AT2G26150 | 200 |
| Les.3551.1.S1_atBH | EU240881 | 5E-113 | Ethylene-responsive transcriptional coactivator multiprotein bridging factor ER24 (LeMBF1); S. lycopersicum | AT3G24500 | 156 |
| Les.3985.1.S1_atBH | AF500011 | 3E-179 | Dehydration responsive-element binding protein (LeDREB1); S. lycopersicum | AT5G05410 | 14 |
| LesAffx.3163.1.S1_at | AT4G12040P | 5E-48 | Zinc finger (AN1-like) family protein; A. thaliana | 10 | |
| Les.2322.1.A1_at | AY044235 | 2E-103 | Jasmonate and ethylene responsive factor 1 (LeJERF1); S. lycopersicum | AT1G53910 | 9 |
| Les.5292.1.S1_at | AT3G27330P | 5E-03 | Zinc finger (C3HC4-type RING finger) family protein; A. thaliana | 8 | |
| Les.2876.2.S1_at | AF208544 | 3E-79 | Heat stress transcription factor A3 (LpHSFA3); L. peruvianum | AT5G03720 | 4 |
| Unclassified | |||||
| LesAffx.15004.1.S1_atBH | AW737975 | cDNA, clone: FC25AB05, HTC in fruit | 380 | ||
| Les.3726.1.S1_atBH | AF204783 | 4E-112 | Ripening-regulated protein DDTFR8; S. lycopersicum | 130 | |
| Les.3822.1.S1_atBH | AF204795 | 1E-33 | Ripening-regulated protein DDTFR17; S. lycopersicum | 55 | |
| Les.1718.1.A1_atBH | AT2G18440P | 6E-03 | Gene with unstable transcript 15 (GUT15); A. thaliana | 50 | |
| Les.195.1.S1_atBH | BM411019 | Transcribed locus | 46 | ||
| Les.5053.1.S1_atBH | BT013184 | Clone 134261F, mRNA sequence | 43 | ||
| Les.1910.1.S1_at | AT2G18440P | 6E-03 | Gene with unstable transcript 15 (GUT15); A. thaliana | 18 | |
| LesAffx.66316.1.S1_at | AT1G24530P | 8E-41 | Transducin family protein/WD-40 repeat family protein; A. thaliana | 10 | |
| Les.4752.1.S1_at | BT013346 | Clone 135282R, mRNA sequence | AT5G63100P | 9 | |
Results are presented as the fold difference between the mean value derived from 12 replicates of heat-stressed versus the mean value derived from 12 replicates of control microspores. Genes found to be statistically significant (P <0.05) are presented in this table.
Either the tomato NCBI accession number, GenBank homologue, or Arabidopsis genome initiative number of best hit from TBLASTX search, using a significance threshold of 1E-4 are given for each Affy ID number.
E-value indicating the significance level of homology to the corresponding gene.
Gene function predicted by the Affymetrix annotation, TIGR definition, and NCBI database.
The Arabidopsis genome initiative number is given for Arabidopsis gene orthologues.
The RMA-normalized ratio of gene expression in heat-stressed (n=12) versus control (n=12) microspores.
To ensure no more than 5% false discovery rate, the BH approach for multiple comparison correction (Benjamini and Hochberg, 1995) was used. Genes that were less than the adjusted P-value of 5% are marked by ‘BH’, the rest (unmarked) are statistically significant (P <0.05, not corrected).
Gene sequences found to be highly expressed in Arabidopsis pollen grains under control conditions (Zimmermann et al., 2004).