Figure 7.

NF-κB and Sp1 bind to the promoter region of Neph3 in cultured human podocytes. In chromatin immunoprecipitation assays, NF-κB and Sp1 antibodies (ab) were used to precipitate DNA-protein complexes from differentiated human podocytes. Real-time PCR was performed with a specific primer pair (shown in Table 1) to amplify extracted DNA fragments. (A) Representative agarose gel: the input lanes confirm the successful PCR, the ab lanes detect the precipitated chromatin, rabbit IgG immunoprecipitation was used as a negative control and H₂O sample represents a control sample that does not contain chromatin. (B) The relative quantity of DNA was counted by comparing the sample fluorescence to the fluorescence values measured from total chromatin input dilution series. Columns indicate the means of two parallel samples. The experiment was repeated twice with similar results.