Table A2.
Primer Sequences for RT-PCR
| Assay | Primer Sequence |
|---|---|
| EEF2 (TaqMan) | |
| EEF2 10(+)a | GAAGCGGCTGGCCAAGTCC |
| EEF2 12(−)a | CGACTCTTCACTGACCGTCTCG |
| EEF2 Probe (BHQ) | CCATGGTGCAGTGCATCATCGAGGAGTCGG |
| TEL-AML | |
| AML4 | CAGAGTGCCATCTGGAACAT |
| TEL5 | AACCTCTCTCATCGGGAAGA |
| TEL-AML2 FLU | GCAGAATGCATACTTGGAATG |
| TEL-AML3 FLU | ATAGCAGATGCCAGCACGAGC |
| TEL-AML4 FLU | ATAGCAGGTGGTGGCCCTAGG |
| BCR-ABL (TaqMan for both B2/3 and E1 forms) | |
| E1(+)A | CTGCCCGGTTGTCGTGTC |
| BCR2(+)a | CTGACCAACTCGTGTGTGAAAC |
| ABL2(−) | CTCAGACCCTGAGGCTCAAAG |
| E1 Probe | CAAGACCGGGCAGATCTGGCCCAAC |
| B2 Probe | CTGTCCACAGCATTCCGCTGACCATCA |
| E2A-PBX | |
| E2A | CTCCACGGCCTGCAGAGTAAG |
| PBX | GCCACGCCTTCCGCTAACA |
| E2A-PBX FLU | ACAGTGTTTTGAGTATCCGAGG |
| MLL-AF4 (nested) | |
| MLL-I | GGTCTCCCAGCCAGCACTGG |
| AF4-I | GCATGGATGACGTTCCTTGCTG |
| MLL-II | GCCTCAGCCACCTACTACAG |
| AF4-II | TTTTGGTTTTGGGTTACAGA |
| MLL FLU | TCCCAAAACCACTCCTAGTGAGC |
| AF4 FLU | GACTCTCAGCATGTCAGTTCTG |
Abbreviations: RT-PCR, reverse transcriptase polymerase chain reaction; EEF2, eukaryotic translation elongation factor 2; TEL, translocation ETS leukemia; AML, acute myeloid leukemia; BCR, break point cluster region; ABL, Abelson murine leukemia viral oncogene; PBX, pre B-cell leukemia transcription factor; MLL, mixed linage leukemia; AF4, ALL1 fused gene from chromosome 1.