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. 2009 May 18;117(9):1414–1419. doi: 10.1289/ehp.0800530

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Alteration of mammary gland morphology by maternal TCDD exposure and HFD. Inguinal mammary whole mounts were made of glands removed at PND36 from progeny of vehicle- and TCDD-treated D2 mice fed HFD or LFD since weaning. Whole mounts were stained with carmine alum and measured for branch elongation (A), number of terminal end buds (B), fat pad length (C), and largest terminal end bud (D). Mean ± SE are shown for LFD groups: vehicle (n = 4 litters) and TCDD (n = 6 litters); and for HFD groups: vehicle (n = 6 litters) and TCDD (n = 5 litters).

*p < 0.05 for diet effects and TCDD × diet effects.