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The effect of IL-10 on expression of Bcl-2 and Bcl-xL mRNA (A) and protein (B) was blocked by LY294002. Cultured cells were treated with 50 μM LY294002 for 30 min followed by exposure to IL-10 (10 ng/ml) for 12 h. Quantitative analysis of the blot results is presented in Supplementary Figures 4 and 5.
