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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Neurochem. 2009 Jun 22;110(5):1409–1421. doi: 10.1111/j.1471-4159.2009.06232.x

Figure 1. Stimulation via glucocorticoid and β2AR receptors synergizes to induce splenocyte apoptosis in vitro.

Figure 1

Naive splenocytes were exposed to increasing concentrations of terbutaline (β2AR agonist) for 24hrs in the presence (open circles; 2µM) or absence (closed circles; 0µM) hydrocortisone (referred to throughout this and subsequent figures as CORT. (A) Apoptosis was analyzed using flow cytometry (Annexin V staining) or (B) DNA laddering. Numbers displayed on the DNA laddering image correspond with the following stimulation conditions: 1:Media; 2:2µM CORT; 3:1mM Terb; 4:10mM Terb; 5:2µM CORT+1mM Terb; 6:2µM CORT+10mM Terb; 7:Staurosporine.