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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Neurochem. 2009 Jun 22;110(5):1409–1421. doi: 10.1111/j.1471-4159.2009.06232.x

Figure 4. CORT increases β2AR expression in lymphocytes after T3 SCI.

Figure 4

(A) Naïve B220+ B cells or (B) B220− cells were incubated in vitro with varying concentrations of CORT for 6hrs. CORT increased β2AR mRNA expression ~20-fold and ~5-fold, respectively. This could be reversed by antagonizing GRs (representative of n=2 independent experiments; RNA pooled from triplicate wells). (C) Using naive splenocytes treated with or without CORT (24 hr pre-treatment) and subsequently stimulated with terbutaline (5hrs before cell harvest), it was confirmed that CORT increases functional β2ARs on lymphocytes. Note the increased intracellular concentrations of cAMP in Terb-stimulated cells that were pre-treated with 2µM CORT; *p<0.05 vs. CORT alone, n=4/group. (D) β2AR mRNA expression is increased in vivo on B cells after T3 but not a T9 SCI (3 days post injury); *p<0.05 vs. laminectomy (Lam) control and T9 SCI. Data were analyzed using a one-way ANOVA with Tukey’s Post-test.