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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Neurochem. 2009 Jun 22;110(5):1409–1421. doi: 10.1111/j.1471-4159.2009.06232.x

Figure 6. CORT and NE synergize to induce Bim expression in lymphocytes in vitro and in vivo after T3 SCI.

Figure 6

(A–C) The combination of CORT and terbutaline signaling induces mRNA (A) and protein (B) expression of the pro-apoptotic molecule, Bim in splenocytes. This effect was blocked by co-treatment with the glucocorticoid receptor antagonist, RU486 (A&C). (D) In vivo following a T3 SCI (3 days post injury), Bim was induced in splenocytes. This induction was inhibited by the combined administration of β2AR and glucocorticoid receptor antagonists (i.e., butoxamine and RU486, respectively). Western blots in B&C show Bim EL isoform and are representative of two independent experiments. n=4/group; *p<0.05 or **p<0.01 vs. media-stimulated (A–C) or sham-injured control (D); One-way ANOVA with Tukey’s Post-test.