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. Author manuscript; available in PMC: 2009 Sep 3.
Published in final edited form as: Neurosci Lett. 2008 Jul 10;442(3):190–194. doi: 10.1016/j.neulet.2008.06.086

Fig. 2.

Fig. 2

Real-time PCR reactions were performed using SYBR green I. The standard curve was generated using plasmid DNA containing the GAPDH cDNA. The amount of the TH mRNA in GFP+ cells was set as 100 to calculate the relative amounts of each mRNA. Each reaction has been performed in triplicate using an identical amount of cDNAs as the template. The expression of TH and LRRK2 was measured as described in Materials and Methods. GAPDH expression levels were used to normalize mRNA levels of each gene. Asterisk indicates statistically significant difference from GFP− cells (*P< 0.01).