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. 2009 Sep 15;4(9):e7020. doi: 10.1371/journal.pone.0007020

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Medendorp et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Endogenous MAD2B, RAN and γ-tubulin (mitotic spindle) are shown in green, red and white, respectively. DAPI staining (blue) was used to mark nuclei and chromosomes. The overlay of the different signals (Merge) reveals a near perfect co-localization of the respective proteins at the mitotic spindle (arrows). Images were captured using confocal laser scanning microscopy.