Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 15;4(9):e7020. doi: 10.1371/journal.pone.0007020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Medendorp et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

COS-1 cells were transiently transfected with MAD2B, blocked at different phases in the cell cycle and, subsequently, released from these cell cycle blocks during different time intervals. (1) cells isolated from a confluent culture, (2) cells isolated 3 h after 1∶2 splitting of a confluent culture, (3) cells blocked with nocodazole, (4) cells blocked with nocodazole and released for 4 h, (5) cells blocked with nocodazole and released for 8 h, (6) cells blocked with hydroxyurea, and (7) cells blocked with hydroxyurea and released for 6 h. (A) FACS profiles confirming the presence of the cells in the different phases of the cell cycle are shown. (B) Western blot analysis of total lysates with anti-RAN, anti-MAD2B and anti-γ-tubulin antibodies (Input) and after anti-RAN immunoprecipitation of the respective lysates (1–7) using an anti-MAD2B antibody (IP).