Table 2. Frequency of Sp1 consensus binding sites in the promoters of genes deregulated by Sp1.
| Sp1 site: GGGGCGGGG | ||||
| Percentage of genes with binding sites | p-value (d) | Number of binding sites per gene | p-value (d) | |
| control list (a) | 15.1% | 1.3 | ||
| up-regulated genes (b) | 12.4% | 0.0715 | 1.2 | 0.2585 |
| down-regulated genes (c) | 20.3% | 0.0031 | 1.7 | 0.0001 |
| Variants of Sp1 site: GG[G/T]G[C/T]GGG | ||||
| Percentage of genes with binding sites | p-value (d) | Number of binding sites per gene | p-value (d) | |
| control list (a) | 39.4% | 1.7 | ||
| up-regulated genes (b) | 41.3% | 0.3591 | 1.7 | 0.82 |
| down-regulated genes (c) | 47.5% | 0.0005 | 2 | 0.0004 |
| STAT6 site: TTCNNNNGAA | ||||
| Percentage of genes with binding sites | p-value (d) | Number of binding sites per gene | p-value (d) | |
| control list (a) | 41.4% | 1.4 | ||
| up-regulated genes (b) | 40.0% | 0.5028 | 1.3 | 1 |
| down-regulated genes (c) | 36.9% | 0.0549 | 1.3 | 0.1694 |
Transcription factor binding sites were searched among the first 2000 nucleotides of the promoter region of differentially expressed genes and non-regulated genes using PATTERNn service. Sp1 binding motifs: GGGGCGGGG and GG[G/T]G[C/T]GGG. STAT6 binding motif: TTCN4GAA.
(a)
Control list of non-regulated genes generated randomly as described in the experimental procedures.
(b)
Up-regulated genes from differentially expressed gene list (>1.3 fold).
(c)
Down-regulated genes from differentially expressed gene list (<1.3 fold).
(d)
Statistical significance calculated as described in the experimental procedures. P-values are the comparison of up-regulated vs control and down-regulated vs control.