Figure 4.

Cancer cell–derived MPs accumulate at the site of injury in mice. (A) Half-life of DiO-labeled Panc02-derived MPs in the blood circulation of a healthy mouse (n = 4 mice, performed independently) reported as the mean ± SD. (B) Imaging of DiD-labeled Panc02-derived MPs using fibered fluorescence microscopy. Panc02-derived MPs were labeled with DiD (left) before infusion into the blood circulation of a living mouse. Although no fluorescent signal was detected in the microcirculation before injury (middle), MPs were detected accumulating at the site of FeCl₃-induced injury (right). Images are representative of six injuries performed in three mice, independently. (C) Intravital videomicroscopy visualization of mesenteric vessels. DiO-labeled MPs (depicted in green; 0.2 µg of MP-associated proteins/g/mouse) and their DiD-labeled parental cells (depicted in red; 5,000 cells/g/mouse) were simultaneously infused into a recipient mouse. Circulating MPs and cells were detected in the microcirculation before injury (second from left). Note that no fluorescent signal was detected before infusion of MPs and cells (left). The experiment was independently performed three times in three mice.