Figure 5.

Ahr has no influence in CpG-ODN signaling pathway. (A) WT and Ahr KO peritoneal macrophages were stimulated with CpG-ODN for 24 h. The production of IL-6 and IL-10 were measured by means of ELISA. Data show means ± SE of three independent experiments. (B) RAW cells were transiently cotransfected with luciferase reporter gene construct of the murine IL-6 promoter and an expression vector for Ahr (RAW/Ahr) or empty control expression vector (RAW/empty vector). 6 h after transfection, cells were stimulated with CpG-ODN for 12 h. Luciferase assay and quantitation were performed as described in Materials and methods. Data show means ± SEM of three independent experiments. Peritoneal macrophages were stimulated with LPS and CpG-ODN. (C) Cells were lysed, immunoprecipitated by Ahr, and analyzed by Western blotting with anti-Stat1 Ab. IP, immunoprecipitation; IB, immunoblot. (D) Whole-cell lysates subjected to Western analysis with anti-pYStat1 antibodies. Data are from one representative of three independent experiments.