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. 2009 Aug 31;206(9):1929–1940. doi: 10.1084/jem.20090896

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© 2009 Agius et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Old endothelium is not defective and can up-regulate adhesion molecules in vitro. Young and old normal skin explants were cultured in RPMI + 10% human serum or in RPMI + 10% human serum supplemented with TNF-α (110,000 U/ml) and IFN-γ (100 U/ml) for 16 h at 37°C and 5% CO_2. Histological sections from skin explants were stained with antibodies to CD31 and E-selectin, ICAM-1, and VCAM-1. The results shown represent one young (A and B) and one old (C and D) subject showing VCAM-1 (red) and CD31 (green) staining. These experiments were performed in a total of three different young and three different old subjects that showed similar results. Bars, 100 µm.