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. 2009 Aug 31;206(9):1941–1955. doi: 10.1084/jem.20090199

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© 2009 Schmitz et al.

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Figure 3. — Inhibition of mast cell degranulation and protection from active systemic anaphylaxis in Qβ–Fel d1-vaccinated mice. (A) Schematic outline of the experiment. (B) Serum of individual mice was collected before (d35) and after (d45) vaccination. Mean Fel d1–specific IgG titers ± SD are shown (n = 7). (C) 11 d after vaccination, all groups were challenged i.v. with rFel d1 and body temperature was measured at regular intervals. Changes in body temperature in degrees celsius ± SD (n = 3) are shown on the left and mean area above the curves ± SD (n = 3) on the right. Individual areas above the curves were determined and compared by one-way analysis of variance (ANOVA) using Bonferroni's post test (PBS/Qβ, NS; PBS/Qβ–Fel d1, P < 0.01; Qβ/Qβ–Fel d1, P < 0.01. (D) Ear prick tests with rFel d1 in naive, PBS-, Qβ-, or Qβ–Fel d1-treated mice. One representative mouse of each group is shown on the left. Mean net intensity of pricked ears ± SD (n = 4) from the densitometric quantification of dye extravasation is shown on the right. (E) OVA/rFel d1–sensitized mice were vaccinated with Qβ or Qβ–Fel d1 and pricked with OVA in the left ear and rFel d1 in the right ear. Mean net intensities ± SD (n = 5) are shown. Qβ versus Qβ–Fel d1/OVA challenge was NS. Net intensity of the Qβ–Fel d1/Fel d1-challenged group is significantly reduced as compared with all other groups. All data shown are representative of four independent experiments with at least the same number of mice per group as shown in A–D. *, P < 0.01; **, P < 0.005; ***, P < 0.0005.