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. Author manuscript; available in PMC: 2010 Aug 19.
Published in final edited form as: J Am Chem Soc. 2009 Aug 19;131(32):11581–11589. doi: 10.1021/ja904318w

Figure 4.

Figure 4

Inhibition of protein splicing by mutations of conserved histidines (B-block histidine H73A, F-block histidine H429A and penultimate histidine H439A). A. SDS-PAGE of in vivo splicing assay.B. Western blot with an anti-C-extein antibody. MW=Molecular weight marker; Ui=Uninduced; MIC=MBP-intein-C-extein fusion (71.5 kD); MC = MBP-C-extein fusion (56.1 kD); M=MBP (43.0 kD); IC-intein-C-extein fusion (28.6 kD) I =intein (15.4 kD).