Figure 1.

Efficiency of 10-23 DNAzyme at different cleavage sites. A series of different cleavage sites within regions A, B and C of the AChR α-subunit target sequence was studied to investigate 10-23 DNAzyme cleavage efficiency. Each panel shows the substrate sequence targeted by each DNAzyme. Cleavage was carried out under simulated physiological conditions of 2 mM Mg²⁺ for four hours. In region A three cleavage sites were targeted (AU, GU and AC). In regions B and C four cleavage sites (AC, GC, GU and AU) were targeted.