Table 1.
DNAzyme nomenclature and target sequences used in this study, with cleavage sites in bold.
| Region | DNAzyme | Target sequence 5′ - 3′ | |||
|---|---|---|---|---|---|
| Arm II | cleavage site | Arm I | |||
| A | 463GU(10+10) | GACGGCUCU | GU | CGUGGCCAU | |
| 463GU(13+9) | CUACGACGGCUCU | GU | CGUGGCCA | ||
| 463GU(9+13) | GACGGCUCU | GU | CGUGGCCAUCAA | ||
| 472AU(10+10) | UGUCAUGGCC | AU | CAACCCGGA | ||
| 472AU(13+9) | CUCUGUCAUGGCC | AU | CAACCCGG | ||
| 472AU(9+13) | GUCAUGGCC | AU | CAACCCGGAAAG | ||
| 476AC(10+10) | AUGGCCAUCA | AC | CCGGAAAGC | ||
| B | 685AC(13+9) | CUCCUUCUUCUUA | AC | UGGCCUGG | |
| 694GU(13+9) | CUUAACUGGCCUG | GU | AUUCUACC | ||
| 696AU(13+9) | UAACUGGCCUGGU | AU | UCUACCUG | ||
| 765GU(13+9) | UGUCUUUGACUGU | GU | UCCUUCUG | ||
| 765GU(9+13) | UUUGACUGU | GU | UCCUUCUGGUCA | ||
| C | 799AC(13+9) | GCUGAUCCCCUCC | AC | GUCCAUUG | |
| 801GU(13+9) | UGAUCCCCUCCAC | GU | CCAUUGCU | ||
| 801GU(9+13) | CCCCUCCAC | GU | CCAUUGCUGUGC | ||
| 805AU(13+9) | CCCCTCCACGTCC | AU | UGCUGUGC | ||
| 808GC(13+9) | CUCCACGUCCAUU | GC | UGUGCCCU | ||
| 811GU(10+10) | GUCCAUUGCU | GU | GCCCUUGAU | ||
| 811GU(13+9) | CACGUCCAUUGCU | GU | GCCCUUGA | ||
| 811GU(9+13) | UCCAUUGCU | GU | GCCCUUGAUUGG | ||
| 811GU(13+13) | CACGUCCAUUGCU | GU | GCCCUUGAUUGG | ||
| Synthetic RNA substrates | 694GU(7+7) | UGGCCUG | GU | AUUCUA | |
| 694AU(7+7) | UGGCCUG | AU | AUUCUA | ||
| 694GC(7+7) | UGGCCUG | GC | AUUCUA | ||
| 694AC(7+7) | UGGCCUG | AC | AUUCUA | ||
| Chemically modified DNAzymes | 463GU(10+10)LNA | CGACGGCUCU | GU | CGUGGCCA | |
| 463GU(10+10)iT | TCGACGGCUCU | GU | CGUGGCCAT | ||