Figure 3.

A siRNA inhibits replication of 5 different strains of HIV-1. Graphs show kinetics of viral replication following transfection of HIV-prom-A siRNA into MAGIC-5 cells 5 hours after infection with laboratory strains of HIV-1 (NL 4-3, IIIB/LAI, and RF) or two clinical isolates (CL-1 and CL-2) from patients failing multiple combination anti-retroviral regimens (Kaufmann et al, 2001). HIV infection was allowed to establish during a 5 hour incubation. Thereafter, cells were transfected with HIV-prom-A siRNA. Infection of MAGIC-5 cells was confirmed by HIV DNA-PCR (data not shown). Reverse transcriptase (RT) levels are shown for cultures transfected with HIV-prom-A siRNA (blue triangle), mock transfected cells (red circle), and uninfected cells (black square). Black arrow indicates timing of transfection.