Figure 4.

A schematic flow chart of an improved siRNA design algorithm. This proposed approach takes into account of the following: 1. Screening for potential target accessibility, 2. Elimination of known immune stimulatory elements (ISEs), 3. Elimination of candidate siRNAs that show high number of potential off-target genes by 5′-seed pairing as identified through algorithms developed for miRNA target prediction. * Most of existing siRNA design algorithms include off-target screening by perfect or near perfect base pairing.