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. 2006 Feb 28;2(1):154–160.

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© Copyright Yusuke Ohnishi et al

This is an open access article, published under the terms of the Licence for Users available at http://www.libpubmedia.co.uk/RNAiJ/LicenceForUsers.pdf. This licence permits noncommercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.

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Figure 3. — Production of A 40 and A 42 peptides under ASP-RNAi. The pAPP695_SWE (A-C) plasmid and both the pAPP695_SWE and pAPP695_WT (D-F) plasmids together with the indicated siRNA duplexes against the Swedish mutant were cotransfected into Cos-7 cells, and expressed sAPP polypeptide and A 40 and A 42 peptides in culture media were examined by western blotting (A, D) and ELISA (B, C, E, F), respectively. “Vector” indicates cells transfected with only plasmid(s). Endogenous and exogenous (expressed) sAPPs are indicated by asterisks and arrow heads, respectively. ELISA data are averages of three independent determinations. Error bars represent standard deviations.