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Effects of Sp proteins on cycle phase distribution in Panc-1 cells. Cells were transfected with Scramble siRNA (iScr, non-specific) or with small inhibitory RNA for iSp1, iSp3 or iSp4, and the percentage distribution of Panc-1 cells in G0/G1, G2/M and S phases of the cell cycle were determined by FACS analysis (Abdelrahim et al, 2004). Sp3 knockdown increased G0/G1 and decreased S phase due to increased expression of p27.
