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Complexity of d-siRNA pool. (A) A 660 bp fragment of the human cyclin B1 dsRNA was digested in vitro with human recombinant Dicer. Sixty of the resulting siRNAs were cloned and sequenced. (B) The predicted frequencies of cloning individual siRNAs once, twice, three times, or four times, out of a sample of 60 clones, for three different assumed pool complexities (c=31, 200, 640). The observed frequencies (open circles) fit best to a pool complexity of 200.
