Skip to main content
. 2006 Jul 17;2(2):181–194.

Figure 2.

Figure 2.

Silencing p38α MAPK expression with synthetic siRNAs, d-siRNAs, and e-siRNAs. (A) Schematic of the p38α MAPK cDNA and the sequences targeted by the siRNAs, d-siRNAs, and e-siRNA employed here. The lengths of the cDNA, siRNAs, and pools are drawn to scale. (B) Dose-dependent decreases in p38α MAPK protein levels in HeLa cells transfected with siRNAs, d-siRNAs, and e-siRNAs. Equivalent amounts of whole cell lysates were subjected to SDS-PAGE and immunoblotted with MEK1 antiserum (a loading control) or a p38α MAPK antibody. (C) Band intensity was quantified, normalized to the respective control (a GL3 luciferase-derived siRNA as the synthetic control, and Dicer- or RNase III-generated luciferase d-siRNAs/e-siRNAs as the other controls), and plotted as dose/inhibition curves.