Table 2.
Global changes in mRNA levels in cells treated with diced siRNAs, RNase-III siRNAs and synthetic siRNAs
| GL3 controls | Diced siRNAs | RNase III | Synthetic siRNAs | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mock | d-GL3 | e-GL3 | si-GL3 | d-14-1 | d-14-5 | d-14-8 | e-14-8 | 14-1 | 14-5 | 14-8 | |
| Number of mRNAs that changed more than 2-fold | 0 | 0 | 0 | 13 | 5 | 4 | 1 | 0 | 1 | 123 | 57 |
| Number of mRNAs that decreased more than p38α MAPK | 1820 | 3653 | 3330 | 3329 | 32 | 23 | 18 | 93 | 47 | 428 | 171 |
| Number of mRNAs that changed significantly by SAM | - | 0±0 | 4±1 | 33±1 | 7±1 | 9±2 | 12±1 | 11±4 | 35±1 | 1105±1 | 322±1 |
A total of 9538 mRNA spots (corresponding to 7587 different genes) were considered well-measured in all 30 siRNA-treated samples. Of those, 7257 spots were also well-measured in the three mock-transfected samples. The ranges shown for the number of mRNAs that changed significantly by SAM are calculated from the estimated false discovery rate.