Table 3.
siRNA seed matches to 3′-UTR can explain some but not all siRNA induced off-target effects.
| siRNA | ||||
|---|---|---|---|---|
| si-GL3 | 14-1 | 14-5 | 14-8 | |
| Number of single seed matches to 3′UTR human genome | 1187 | 1463 | 4460 | 4967 |
| Number of multiple seed matches to 3′UTR human genome | 55 | 124 | 927 | 1012 |
| Number of mRNAs that were significantly reduced (SAM) | 34 | 35 | 239 | 216 |
| Number of SAM mRNAs with single seed match | 3 | 3 | 42 | 47 |
| Number of random mRNAs with single seed match | 1 | 1 | 19 | 20 |
| P-value (χ2 test) | 1 | 1 | 0.01 | 0.001 |
| Number of SAM mRNAs with multiple seed match | 0 | 0 | 10 | 16 |
| Number of random mRNAs with multiple seed match | 0 | 0 | 7 | 4 |
| P-value (χ2 test) | 1 | 1 | 1 | 0.01 |