Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep;134(3):243–258. doi: 10.1085/jgp.200910255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Liu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Extracellular protons reduce single-channel conductance of TRPV1 channels. (A) Representative single-channel currents at the indicated pH recorded from an outside-out patch of oocyte membrane expressing TRPV1. The external and internal solutions were symmetrical 150 mM KCl. Recordings at two membrane potentials were shown (±60 mV) and were low-pass filtered at 2 kHz. (B) All-point amplitude histograms of single-channel currents. The histograms were fit to sums of two Gaussian functions to determine the closed and open amplitudes. The solid lines represent best fits. (C) Dose–response curves for the unitary current amplitudes obtained at ±60 mV versus the extracellular pH. The solid lines represent fits to the Hill equation with pKa ∼ 7, Hill coefficient of n ∼ 0.6 for V_h = −60 mV, and pKa ∼ 6 and n ∼ 0.3 for V_h = +60 mV, respectively. (D) Normalized dose–response relationships showing different dependence on pH at different membrane potentials (±60 mV).