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. 2008 May 27;4(1):281–288.

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©The Authors

This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.

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Figure 1. — Electric field intensity effect on permeabilization and viability. Percentages of permeabilized cells, cell viability and viable permeabilized cells were plotted as a function of the electric field intensity. Permeabilization was assayed by the penetration of PI in cells and analyzed by flow cytometry (▲). The live cells were determined 24 hrs after the treatment by crystal violet coloration (■). Error bars represent standard deviation. The percentage of viable permeabilized cells were determined by calculating (permeabilization (%)+viability(%) −100).