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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Cancer Res. 2009 May 19;69(11):4613–4620. doi: 10.1158/0008-5472.CAN-08-2816

Figure 2.

Figure 2

Activation of FGFR1 regulates proliferation and survival. A, Immunoprecipitation of FGFR1 from NHUC-FR1 cultured with FGF2 for 15, 30 and 60 minutes. Blots were probed with an anti-phosphotyrosine antibody (pTYR) and reprobed with anti-FGFR1 antibody as loading control. B, NHUC-VEC (dashed line) and NHUC-FR1 (solid line) were grown in supplement-free medium with heparin and FGF2 (10ng/ml). C, NHUC-VEC and NHUC-FR1 were grown in supplement-free medium with heparin (black bar), or heparin and FGF2 (grey bar) for 24 hours prior to addition of BrdU. BrdU incorporation was measured by FACS. D, NHUC-VEC and NHUC-FR1 were grown in supplement-free medium for 96 hours in the presence of heparin (black bar), or heparin and FGF2 (grey bar). Viability and apoptosis were analysed by FACS.