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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Cancer Res. 2009 May 19;69(11):4613–4620. doi: 10.1158/0008-5472.CAN-08-2816

Figure 4.

Figure 4

MAPK activation regulates proliferation and survival. A, NHUC-FR1 and NHUC-Y766F were cultured with heparin (dark grey), FGF2 (black), FGF2 and U0126 (white), or FGF2 and PD98059 (light grey) for 24 hours prior to addition of BrdU. BrdU incorporation was analysed using FACS. B, Cells were cultured in supplement-free medium with heparin and FGF2 for 96 hours. Apoptosis was analysed by AnnexinV staining. C, NHUC-VEC (black bar), NHUC-FR1 (white bar) and NHUC-Y766F (grey bar) were cultured with heparin, FGF2 and U0126 or PD98059 for 96 hours. D, NHUC-VEC, NHUC-FR1 and NHUC-Y766F were cultured with heparin and FGF2 for indicated times and their lysates blotted for cyclinD1, MCL1 and phosphorylated BAD. E, NHUC-FR1 were cultured with indicated substances for 4 hours and their lysates blotted for cyclin D1, MCL1 and phosphorylated BAD.