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Roscovitine regulates TNFα-mediated NFκB activation. Purified T cells were treated with TNFα (100 ng/ml) in the absence or the presence of 12 μM roscovitine for indicated time points. Cytoplasmic cell lysates were prepared and effects of roscovitine on TNFα induced phosphorylation of IkBa and phosphorylation of Ser536 of p65 were analyzed by immunoblotting with specific antibodies.
