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. Author manuscript; available in PMC: 2009 Sep 3.
Published in final edited form as: Nat Neurosci. 2009 Jan 11;12(2):113–115. doi: 10.1038/nn.2244

Figure 2.

Figure 2

Developmental regulation of proBDNF and p75 expression. (a) Immunofluorescence detection of BDNF in mouse hippocampi (P28) using mAb287, antibodies to HA and Cy3-conjugated streptavidin, shown in serial sections of Bdnf-HA/+ or wild-type brain. Non-immune mouse IgG was used as a control. Scale bars represent 250 µm. (b) Hippocampal lysates from mice of the indicated genotype and ages were subjected to immunoprecipitation/western blot analysis for BDNF isoforms (top), probed to detect p75 (middle) or probed for ERK as a loading control (bottom). (e) Immunofluorescence detection of proBDNF or p75 using 1-week-old and 4-week-old mouse brains. Non-immune IgG was used as a control. Scale bars represent 250 µm.