Table 3. Analysis of cytokines and chemokines from wild-type and Cav-1 null macrophages^a.

Cav-1 null (KO) macrophages infected with T. cruzi trypomastigotes display reduced secretion of chemokines and cytokines. Peritoneal macrophages were infected with culture-derived trypomastigotes at a 2:1 parasite/host cell ratio. Cultures were maintained at 37°C for 24 hrs. Supernatants were collected and chemokine and cytokine levels from wild-type and Cav-1 null macrophages were determined. The bars represent mean ± standard deviation of triplicate counts.

Sample	CCL3	CCL2	CXCL1	CCL5	G-CSF	TNF-α	CXCL10	IL-1α	IL-6
WT Infected	73.75 ±12	776.1 ± 50	491 ± 20	14.5 ± 3	231 ± 16	26.5 ± 2	193.4 ± 7	30 ± 1	72.2 ± 1
KO Infected	65.25 ± 7	327.9 ± 56	77.6 ± 3	3.7 ± 0.2	25.1 ± 2	14.2 ± 0.5	83.1 ± 15	3.41 ± 0.1	7.5 ± 1
p value	0.426	0.014*	0.001*	0.028*	0.003*	0.011*	0.011*	0.002*	0.003*

^aConcentrations are [pg/ml]

^*p < 0.05