Table II.
Calculated Enhancement Factor for Labeled Avidin
Probe | Gexc | Γ (ns−1)a | Γm(ns−1)a | Nr | ||||
---|---|---|---|---|---|---|---|---|
Pacific Blue | 28 | 235 | 25 | 0.32 | 11b | 35 | ||
Lissamine | 16 | 200c | 9.5c | 0.53 | 23b | 45 | ||
Texas Red | 46 | 200c | 418c | 0.16 | 71b | 455 |
Calculated using Γ = Q/〈τ〉.
Calculated assuming the quantum yield =1.0 on the silver island films.
Calculated assuming two-photon excitation displays a 200-fold increase in probe fluorescence on the silver island films.