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. 2009 Sep 3;106(38):16511–16516. doi: 10.1073/pnas.0902743106

Fig. 2.

Fig. 2.

O-1602 and LPI stimulate human osteoclast polarization and resorption. Human osteoclasts cultured on dentine discs were treated with vehicle (control) or 1 nM to 1 μM O-1602 with or without 500 nM CBD for 5 days. (A) The number of F-actin rings is expressed as percentage of control cultures ± SEM. Black bars represent O-1602 alone (n = 5 experiments with 5 replicates for each); white bars represent O-1602 + 500 nM CBD (n = 4 experiments with 4 or 5 replicates for each). ANOVA with Bonferroni post-test; *, P < 0.05; **, P < 0.01 compared with control; #, P < 0.05; ###, P < 0.001 compared with O-1602 alone. (B) Resorption area is expressed as percentage of control ± SEM. Black bars represent O-1602 alone (n = 5 experiments with 5 replicates for each); white bars represent O-1602 + 500 nM CBD (n = 4 experiments with 4 or 5 replicates for each). ANOVA with Bonferroni post-test; *, P < 0.05 compared with control; ###, P < 0.001 compared with O-1602 alone. (C) Representative images of human osteoclasts cultured on dentine, after treatment with vehicle (control), 50 nM O-1602, or 50 nM O-1602 + 500 nM CBD. Cells were stained to visualize polarized cells with F-actin rings (Inset). (D) Human osteoclasts on dentine discs were treated with vehicle (control) or 1 nM to 1 μM LPI for 5 days. The number of F-actin rings is expressed as percentage of control cultures ± SEM (n = 4 experiments with 4 or 5 replicates for each). ANOVA with Dunnett's post-test; *, P < 0.05; **, P < 0.01 compared with control. (E) Resorption area is expressed as percentage of control ± SEM. (n = 4 experiments with 3–5 replicates for each). ANOVA with Dunnett's post-test; **, P < 0.01.