CyRP peptides bind to Cx43CT and interfere with octanol-induced uncoupling of Cx43 channels. A: Surface plasmon resonance results. Change in angle of incidence of resonance (“resonance units”) plotted against time after onset of peptide exposure. Upward and downward arrows indicate onset and washout of CyRP-62 superfusion. B–D: Patch clamp results from N2A cells expressing Cx43. Peptides were diluted in patch pipettes (100 μM). Time “zero:” onset of octanol superfusion. Red and black symbols indicate, respectively, results from cells exposed to a given peptide, or kept as control. Significance values were p<0.001, p=0.148 and p=0.019 for data in panels B, C and D, respectively (each data set compared to results obtained without peptide).