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. Author manuscript; available in PMC: 2010 Jul 17.
Published in final edited form as: Circ Res. 2009 Jun 25;105(2):176–184. doi: 10.1161/CIRCRESAHA.109.200576

Figure 6.

Figure 6

CyRP-71 did not prevent octanol-induced uncoupling in N2a cells expressing Cx43 mutant M257 (panel A) or an alternative connexin isotype, Cx40 (panel B). CyRP-71 prevented acidification-induced uncoupling of Cx43-expressing N2a cells (panel C). CyRP-71 (0.1 mmol/L) was diluted in internal pipette solution buffered to pH 6.2. In the absence of peptide, Gj decreased to 17.3 ± 1.7% of initial value. In the presence of CyRP-71, Gj decreased only to 49.0 ± 5.9% of maximum (p=0.002). Panel D: CyRP-71 prevented acidification-induced uncoupling of neonatal rat ventricular gap junctions (pH 6.2). Fifteen minutes after patch break, Gj in control and in the presence of linear peptide RRPPYR decreased to 8.4 ± 3.2% and 16.6 ± 6.5% of initial value, respectively (p value RRPPYR=0.48 when compared to control). In the presence of CyRP-71 (0.1 mmol/L), Gj decreased only to 47.0 ± 9.5% of maximum (p=0.006).