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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Eur J Pharmacol. 2009 May 20;615(1-3):144–149. doi: 10.1016/j.ejphar.2009.05.007

Fig. 1. Experimental protocol.

Fig. 1

Bovine pulmonary arterial endothelial cells were exposed to or not exposed to a 3-h oxygen-glucose deprivation (OGD) and posttreated with or without 2% isoflurane (Iso-post) for 1 h immediately after the oxygen-glucose deprivation in the presence or absence of various reagents (R), such as glybenclamide, 5-hydroxydecanoate or chelerythrine. In some cells, 100 μM diazoxide (Di) was applied for 1 h immediately after oxygen-glucose deprivation in the presence or absence of reagents (R), such as 5-hydroxydecanoate or chelerythrine. Lactate dehydrogenase released into incubation buffer was determined 3 h after oxygen-glucose deprivation.